The Chlamydia-Secreted Protease CPAF Promotes Chlamydial Survival in the Mouse Lower Genital Tract.

Journal Article (Journal Article)

Despite the extensive in vitro characterization of CPAF (chlamydial protease/proteasome-like activity factor), its role in chlamydial infection and pathogenesis remains unclear. We now report that a Chlamydia trachomatis strain deficient in expression of CPAF (L2-17) is no longer able to establish a successful infection in the mouse lower genital tract following an intravaginal inoculation. The L2-17 organisms were cleared from the mouse lower genital tract within a few days, while a CPAF-sufficient C. trachomatis strain (L2-5) survived in the lower genital tract for more than 3 weeks. However, both the L2-17 and L2-5 organisms maintained robust infection courses that lasted up to 4 weeks when they were directly delivered into the mouse upper genital tract. The CPAF-dependent chlamydial survival in the lower genital tract was confirmed in multiple strains of mice. Thus, we have demonstrated a critical role of CPAF in promoting C. trachomatis survival in the mouse lower genital tracts. It will be interesting to further investigate the mechanisms of the CPAF-dependent chlamydial pathogenicity.

Full Text

Duke Authors

Cited Authors

  • Yang, Z; Tang, L; Shao, L; Zhang, Y; Zhang, T; Schenken, R; Valdivia, R; Zhong, G

Published Date

  • September 2016

Published In

Volume / Issue

  • 84 / 9

Start / End Page

  • 2697 - 2702

PubMed ID

  • 27382018

Pubmed Central ID

  • PMC4995919

Electronic International Standard Serial Number (EISSN)

  • 1098-5522

Digital Object Identifier (DOI)

  • 10.1128/IAI.00280-16


  • eng

Conference Location

  • United States