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Rapid Reprogramming of Primary Human Astrocytes into Potent Tumor-Initiating Cells with Defined Genetic Factors.

Publication ,  Journal Article
Li, F; Liu, X; Sampson, JH; Bigner, DD; Li, C-Y
Published in: Cancer Res
September 1, 2016

Cancer stem-like cells (CSC) are thought to drive brain cancer, but their cellular and molecular origins remain uncertain. Here, we report the successful generation of induced CSC (iCSC) from primary human astrocytes through the expression of defined genetic factors. Combined transduction of four factors, Myc, Oct-4, p53DD, and Ras, induced efficient transformation of primary human astrocytes into malignant cells with powerful tumor-initiating capabilities. Notably, transplantation of 100 transduced cells into nude mice was sufficient for tumor formation. The cells showed unlimited self-renewal ability with robust telomerase activities. In addition, they expressed typical glioma stem-like cell markers, such as CD133, CD15, and CD90. Moreover, these cells could form spheres in culture and differentiate into neuron-like, astrocyte-like, and oligodendrocyte-like cells. Finally, they also displayed resistance to the widely used brain cancer drug temozolomide. These iCSCs could provide important tools for studies of glioma biology and therapeutics development. Cancer Res; 76(17); 5143-50. ©2016 AACR.

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Published In

Cancer Res

DOI

EISSN

1538-7445

Publication Date

September 1, 2016

Volume

76

Issue

17

Start / End Page

5143 / 5150

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplastic Stem Cells
  • Mice, Nude
  • Mice
  • Humans
  • Heterografts
  • Flow Cytometry
  • Cellular Reprogramming Techniques
  • Cellular Reprogramming
 

Citation

APA
Chicago
ICMJE
MLA
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Li, F., Liu, X., Sampson, J. H., Bigner, D. D., & Li, C.-Y. (2016). Rapid Reprogramming of Primary Human Astrocytes into Potent Tumor-Initiating Cells with Defined Genetic Factors. Cancer Res, 76(17), 5143–5150. https://doi.org/10.1158/0008-5472.CAN-16-0171
Li, Fang, Xinjian Liu, John H. Sampson, Darell D. Bigner, and Chuan-Yuan Li. “Rapid Reprogramming of Primary Human Astrocytes into Potent Tumor-Initiating Cells with Defined Genetic Factors.Cancer Res 76, no. 17 (September 1, 2016): 5143–50. https://doi.org/10.1158/0008-5472.CAN-16-0171.
Li F, Liu X, Sampson JH, Bigner DD, Li C-Y. Rapid Reprogramming of Primary Human Astrocytes into Potent Tumor-Initiating Cells with Defined Genetic Factors. Cancer Res. 2016 Sep 1;76(17):5143–50.
Li, Fang, et al. “Rapid Reprogramming of Primary Human Astrocytes into Potent Tumor-Initiating Cells with Defined Genetic Factors.Cancer Res, vol. 76, no. 17, Sept. 2016, pp. 5143–50. Pubmed, doi:10.1158/0008-5472.CAN-16-0171.
Li F, Liu X, Sampson JH, Bigner DD, Li C-Y. Rapid Reprogramming of Primary Human Astrocytes into Potent Tumor-Initiating Cells with Defined Genetic Factors. Cancer Res. 2016 Sep 1;76(17):5143–5150.

Published In

Cancer Res

DOI

EISSN

1538-7445

Publication Date

September 1, 2016

Volume

76

Issue

17

Start / End Page

5143 / 5150

Location

United States

Related Subject Headings

  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplastic Stem Cells
  • Mice, Nude
  • Mice
  • Humans
  • Heterografts
  • Flow Cytometry
  • Cellular Reprogramming Techniques
  • Cellular Reprogramming