Envelope-specific B-cell populations in African green monkeys chronically infected with simian immunodeficiency virus.
African green monkeys (AGMs) are natural primate hosts of simian immunodeficiency virus (SIV). Interestingly, features of the envelope-specific antibody responses in SIV-infected AGMs are distinct from that of HIV-infected humans and SIV-infected rhesus monkeys, including gp120-focused responses and rapid development of autologous neutralization. Yet, the lack of genetic tools to evaluate B-cell lineages hinders potential use of this unique non-human primate model for HIV vaccine development. Here we define features of the AGM Ig loci and compare the proportion of Env-specific memory B-cell populations to that of HIV-infected humans and SIV-infected rhesus monkeys. AGMs appear to have a higher proportion of Env-specific memory B cells that are mainly gp120 directed. Furthermore, AGM gp120-specific monoclonal antibodies display robust antibody-dependent cellular cytotoxicity and CD4-dependent virion capture activity. Our results support the use of AGMs to model induction of functional gp120-specific antibodies by HIV vaccine strategies.
Zhang, R; Martinez, DR; Nguyen, QN; Pollara, J; Arifin, T; Stolarchuk, C; Foulger, A; Amos, JD; Parks, R; Himes, JE; Wang, M; Edwards, RW; Trama, AM; Vandergrift, N; Colvin, L; Dewar, K; Juretic, N; Wasserscheid, J; Ferrari, G; Liao, H-X; Permar, SR
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