Synthesis and secretion of alkaline phosphatase in vitro from first-trimester and term human placentas

Journal Article

The synthesis and secretion of alkaline phosphatases in vitro by human placental tissue incubated in organ culture were studied. First-trimester placenta synthesizes and secretes two different alkaline phosphatase isoenzymes (heat-labile and heat-stable), whereas in term placenta nearly all the alkaline phosphatase synthesized and secreted is heat-stable. The specific activities of alkaline phosphatases in first-trimester and term placental tissue remain constant throughout the time course of incubation. In the media, specific activities increase with time. Hence, alkaline phosphatase synthesis seems to be the driving force for its own secretion. The rates of synthesis de novo and of alkaline phosphatases were measured. The specific radioactivities of the secreted alkaline phosphatases were higher than the corresponding specific radioactivities in the tissue throughout the entire incubation period. The intracellular distribution of the alkaline phosphatase isoenzymes was compared.

Full Text

Duke Authors

Cited Authors

  • Galski, H; Fridovich, SE; Weinstein, D; De Groot, N; Segal, S; Folman, R; Hochberg, AA

Published Date

  • March 15, 1981

Published In

Volume / Issue

  • 194 / 3

Start / End Page

  • 857 - 866

Published By

International Standard Serial Number (ISSN)

  • 0264-6021

Digital Object Identifier (DOI)

  • 10.1042/bj1940857

Language

  • en