Repeatability and reproducibility of optic nerve head perfusion measurements using optical coherence tomography angiography.
Optical coherence tomography angiography (OCTA) has increasingly become a clinically useful technique in ophthalmic imaging. We evaluate the repeatability and reproducibility of blood perfusion in the optic nerve head (ONH) measured using optical microangiography (OMAG)-based OCTA. Ten eyes from 10 healthy volunteers are recruited and scanned three times with a 68-kHz Cirrus HD-OCT 5000-based OMAG prototype system (Carl Zeiss Meditec Inc., Dublin, California) centered at the ONH involving two separate visits within six weeks. Vascular images are generated with OMAG processing by detecting the differences in OCT signals between consecutive B-scans acquired at the same retina location. ONH perfusion is quantified as flux, vessel area density, and normalized flux within the ONH for the prelaminar, lamina cribrosa, and the full ONH. Coefficient of variation (CV) and intraclass correlation coefficient (ICC) are used to evaluate intravisit and intervisit repeatability, and interobserver reproducibility. ONH perfusion measurements show high repeatability [CV≤3.7% (intravisit) and ≤5.2% (intervisit)] and interobserver reproducibility (ICC≤0.966) in all three layers by three metrics. OCTA provides a noninvasive method to visualize and quantify ONH perfusion in human eyes with excellent repeatability and reproducibility, which may add additional insight into ONH perfusion in clinical practice.
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Related Subject Headings
- Tomography, Optical Coherence
- Reproducibility of Results
- Optics
- Optic Disk
- Humans
- Angiography
- 5102 Atomic, molecular and optical physics
- 4003 Biomedical engineering
- 3212 Ophthalmology and optometry
- 1113 Opthalmology and Optometry
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tomography, Optical Coherence
- Reproducibility of Results
- Optics
- Optic Disk
- Humans
- Angiography
- 5102 Atomic, molecular and optical physics
- 4003 Biomedical engineering
- 3212 Ophthalmology and optometry
- 1113 Opthalmology and Optometry