Heteromeric assembly of acid-sensitive ion channel and epithelial sodium channel subunits.

Journal Article

Amiloride-sensitive ion channels are formed from homo- or heteromeric combinations of subunits from the epithelial Na+ channel (ENaC)/degenerin superfamily, which also includes the acid-sensitive ion channel (ASIC) family. These channel subunits share sequence homology and topology. In this study, we have demonstrated, using confocal fluorescence resonance energy transfer microscopy and co-immunoprecipitation, that ASIC and ENaC subunits are capable of forming cross-clade intermolecular interactions. We have also shown that combinations of ASIC1 with ENaC subunits exhibit novel electrophysiological characteristics compared with ASIC1 alone. The results of this study suggest that heteromeric complexes of ASIC and ENaC subunits may underlie the diversity of amiloride-sensitive cation conductances observed in a wide variety of tissues and cell types where co-expression of ASIC and ENaC subunits has been observed.

Full Text

Duke Authors

Cited Authors

  • Meltzer, RH; Kapoor, N; Qadri, YJ; Anderson, SJ; Fuller, CM; Benos, DJ

Published Date

  • August 2007

Published In

Volume / Issue

  • 282 / 35

Start / End Page

  • 25548 - 25559

PubMed ID

  • 17613525

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.m703825200

Language

  • eng