Molecular proximity of cystic fibrosis transmembrane conductance regulator and epithelial sodium channel assessed by fluorescence resonance energy transfer.

Published

Journal Article

We present the evidence for a direct physical association of cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC), two major ion channels implicated in the pathophysiology of cystic fibrosis, a devastating inherited disease. We employed fluorescence resonance energy transfer, a distance-dependent imaging technique with capability to detect molecular complexes with near angstrom resolution, to estimate the proximity of CFTR and ENaC, an essential variable for possible physical interaction to occur. Fluorescence resonance energy transfer studies were complemented with a classic biochemical approach: coimmunoprecipitation. Our results place CFTR and ENaC within reach of each other, suggestive of a direct interaction between these two proteins.

Full Text

Duke Authors

Cited Authors

  • Berdiev, BK; Cormet-Boyaka, E; Tousson, A; Qadri, YJ; Oosterveld-Hut, HMJ; Hong, JS; Gonzales, PA; Fuller, CM; Sorscher, EJ; Lukacs, GL; Benos, DJ

Published Date

  • December 14, 2007

Published In

Volume / Issue

  • 282 / 50

Start / End Page

  • 36481 - 36488

PubMed ID

  • 17913705

Pubmed Central ID

  • 17913705

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M708089200

Language

  • eng

Conference Location

  • United States