N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.

Journal Article (Letter)

N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

Full Text

Duke Authors

Cited Authors

  • Guan, Z; Delago, A; Nußbaum, P; Meyer, B; Albers, S-V; Eichler, J

Published Date

  • September 2016

Published In

Volume / Issue

  • 590 / 18

Start / End Page

  • 3168 - 3178

PubMed ID

  • 27490243

Pubmed Central ID

  • PMC5039095

Electronic International Standard Serial Number (EISSN)

  • 1873-3468

Digital Object Identifier (DOI)

  • 10.1002/1873-3468.12341


  • eng

Conference Location

  • England