Developmental toxicity from exposure to various forms of mercury compounds in medaka fish (Oryzias latipes) embryos.

Published

Journal Article

This study examined developmental toxicity of different mercury compounds, including some used in traditional medicines. Medaka (Oryzias latipes) embryos were exposed to 0.001-10 µM concentrations of MeHg, HgCl2, α-HgS (Zhu Sha), and β-HgS (Zuotai) from stage 10 (6-7 hpf) to 10 days post fertilization (dpf). Of the forms of mercury in this study, the organic form (MeHg) proved the most toxic followed by inorganic mercury (HgCl2), both producing embryo developmental toxicity. Altered phenotypes included pericardial edema with elongated or tube heart, reduction of eye pigmentation, and failure of swim bladder inflation. Both α-HgS and β-HgS were less toxic than MeHg and HgCl2. Total RNA was extracted from survivors three days after exposure to MeHg (0.1 µM), HgCl2 (1 µM), α-HgS (10 µM), or β-HgS (10 µM) to examine toxicity-related gene expression. MeHg and HgCl2 markedly induced metallothionein (MT) and heme oxygenase-1 (Ho-1), while α-HgS and β-HgS failed to induce either gene. Chemical forms of mercury compounds proved to be a major determinant in their developmental toxicity.

Full Text

Duke Authors

Cited Authors

  • Dong, W; Liu, J; Wei, L; Jingfeng, Y; Chernick, M; Hinton, DE

Published Date

  • January 2016

Published In

Volume / Issue

  • 4 /

Start / End Page

  • e2282 -

PubMed ID

  • 27635309

Pubmed Central ID

  • 27635309

Electronic International Standard Serial Number (EISSN)

  • 2167-8359

International Standard Serial Number (ISSN)

  • 2167-8359

Digital Object Identifier (DOI)

  • 10.7717/peerj.2282

Language

  • eng