Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis.

Journal Article (Journal Article)

In most Gram-negative pathogens, the hydrolysis of UDP-2,3-diacylglucosamine to generate lipid X in lipid A biosynthesis is catalysed by the membrane-associated enzyme LpxH. We report the crystal structure of LpxH in complex with its product, lipid X, unveiling a unique insertion lid above the conserved architecture of calcineurin-like phosphoesterases. This structure reveals elaborate interactions surrounding lipid X and provides molecular insights into the substrate selectivity, catalysis and inhibition of LpxH.

Full Text

Duke Authors

Cited Authors

  • Cho, J; Lee, C-J; Zhao, J; Young, HE; Zhou, P

Published Date

  • August 15, 2016

Published In

Volume / Issue

  • 1 / 11

Start / End Page

  • 16154 -

PubMed ID

  • 27780190

Pubmed Central ID

  • PMC5081216

Electronic International Standard Serial Number (EISSN)

  • 2058-5276

Digital Object Identifier (DOI)

  • 10.1038/nmicrobiol.2016.154


  • eng

Conference Location

  • England