Antibiotics as a selective driver for conjugation dynamics.

Journal Article

It is generally assumed that antibiotics can promote horizontal gene transfer. However, because of a variety of confounding factors that complicate the interpretation of previous studies, the mechanisms by which antibiotics modulate horizontal gene transfer remain poorly understood. In particular, it is unclear whether antibiotics directly regulate the efficiency of horizontal gene transfer, serve as a selection force to modulate population dynamics after such gene transfer has occurred, or both. Here, we address this question by quantifying conjugation dynamics in the presence and absence of antibiotic-mediated selection. Surprisingly, we find that sublethal concentrations of antibiotics from the most widely used classes do not significantly increase the conjugation efficiency. Instead, our modelling and experimental results demonstrate that conjugation dynamics are dictated by antibiotic-mediated selection, which can both promote and suppress conjugation dynamics. Our findings suggest that the contribution of antibiotics to the promotion of horizontal gene transfer may have been overestimated. These findings have implications for designing effective antibiotic treatment protocols and for assessing the risks of antibiotic use.

Full Text

Duke Authors

Cited Authors

  • Lopatkin, AJ; Huang, S; Smith, RP; Srimani, JK; Sysoeva, TA; Bewick, S; Karig, DK; You, L

Published Date

  • April 11, 2016

Published In

Volume / Issue

  • 1 / 6

Start / End Page

  • 16044 -

PubMed ID

  • 27572835

Electronic International Standard Serial Number (EISSN)

  • 2058-5276

International Standard Serial Number (ISSN)

  • 2058-5276

Digital Object Identifier (DOI)

  • 10.1038/nmicrobiol.2016.44

Language

  • eng