Nociceptor-Enriched Genes Required for Normal Thermal Nociception.

Published

Journal Article

Here, we describe a targeted reverse genetic screen for thermal nociception genes in Drosophila larvae. Using laser capture microdissection and microarray analyses of nociceptive and non-nociceptive neurons, we identified 275 nociceptor-enriched genes. We then tested the function of the enriched genes with nociceptor-specific RNAi and thermal nociception assays. Tissue-specific RNAi targeted against 14 genes caused insensitive thermal nociception while targeting of 22 genes caused hypersensitive thermal nociception. Previously uncategorized genes were named for heat resistance (i.e., boilerman, fire dancer, oven mitt, trivet, thawb, and bunker gear) or heat sensitivity (firelighter, black match, eucalyptus, primacord, jet fuel, detonator, gasoline, smoke alarm, and jetboil). Insensitive nociception phenotypes were often associated with severely reduced branching of nociceptor neurites and hyperbranched dendrites were seen in two of the hypersensitive cases. Many genes that we identified are conserved in mammals.

Full Text

Duke Authors

Cited Authors

  • Honjo, K; Mauthner, SE; Wang, Y; Skene, JHP; Tracey, WD

Published Date

  • July 12, 2016

Published In

Volume / Issue

  • 16 / 2

Start / End Page

  • 295 - 303

PubMed ID

  • 27346357

Pubmed Central ID

  • 27346357

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2016.06.003

Language

  • eng

Conference Location

  • United States