Identification of microRNAs in Throat Swab as the Biomarkers for Diagnosis of Influenza.


Journal Article

Influenza is a serious worldwide disease that captures global attention in the past few years after outbreaks. The recent discoveries of microRNA (miRNA) and its unique expression profile in influenza patients have offered a new method for early influenza diagnosis. The aim of this study was to examine the utility of miRNAs for the diagnosis of influenza.Thirteen selected miRNAs were investigated with the hosts' throat swabs (25 H1N1, 20 H3N2, 20 influenza B and 21 healthy controls) by real-time quantitative polymerase chain reaction (RT-qPCR) using U6 snRNA as endogenous control for normalization, and receiver operating characteristic (ROC) curve/Area under curve (AUC) for analysis.miR-29a-3p, miR-30c-5p, miR-34c-3p and miR-181a-5p are useful biomarkers for influenza A detection; and miR-30c-5p, miR-34b-5p, miR-205-5p and miR-449b-5p for influenza B detection. Also, use of both miR-30c-5p and miR-34c-3p (AUC=0.879); and miR-30c-5p and miR-449b-5p (AUC=0.901) are better than using one miRNA to confirm influenza A and influenza B infection, respectively.Given its simplicity, non-invasiveness and specificity, we found that the throat swab-derived miRNAs miR-29a-3p, miR-30c-5p, miR-34b-5p, miR-34c-3p, miR-181a-5p, miR-205-5p and miR-449b-5p are a useful tool for influenza diagnosis on influenza A and B.

Full Text

Cited Authors

  • Peng, F; He, J; Loo, JFC; Yao, J; Shi, L; Liu, C; Zhao, C; Xie, W; Shao, Y; Kong, SK; Gu, D

Published Date

  • January 25, 2016

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • 77 - 84

PubMed ID

  • 26917988

Pubmed Central ID

  • 26917988

Electronic International Standard Serial Number (EISSN)

  • 1449-1907

International Standard Serial Number (ISSN)

  • 1449-1907

Digital Object Identifier (DOI)

  • 10.7150/ijms.13301


  • eng