Energy driven cascade recognition for selective detection of nucleic acids with high discrimination factor at room temperature.


Journal Article

In this article, we demonstrated a cascade recognition strategy for the detection of single strand nucleic acid with high discrimination factor at room temperature. The cascade recognition strategy contains a toehold mediated strand displacement and a double-toehold mediated double strand displacement reaction, thus enable the high ability to discern point mutation of target. The discrimination factor of the model target is between 45 and 109, with the medium of 70. This strategy is homogeneous, easy operation, enzyme-free, isothermal, and can be easily adapted to high-throughput devices without the need of designing complicated instruments.

Full Text

Cited Authors

  • Zhang, Z; Li, JL; Yao, J; Wang, T; Yin, D; Xiang, Y; Chen, Z; Xie, G

Published Date

  • May 2016

Published In

Volume / Issue

  • 79 /

Start / End Page

  • 488 - 494

PubMed ID

  • 26745796

Pubmed Central ID

  • 26745796

Electronic International Standard Serial Number (EISSN)

  • 1873-4235

International Standard Serial Number (ISSN)

  • 0956-5663

Digital Object Identifier (DOI)

  • 10.1016/j.bios.2015.12.093


  • eng