Fabrication of Cell Patches Using Biodegradable Scaffolds with a Hexagonal Array of Interconnected Pores (SHAIPs).


Journal Article

Cell patches are widely used for healing injuries on the surfaces or interfaces of tissues such as those of epidermis and myocardium. Here we report a novel type of porous scaffolds made of poly(D,L-lactic-co-glycolic acid) for fabricating cell patches. The scaffolds have a single layer of spherical pores arranged in a unique hexagonal pattern and are therefore referred to as "scaffolds with a hexagonal array of interconnected pores (SHAIPs)". SHAIPs contain both uniform pores and interconnecting windows that can facilitate the exchange of biomacromolecules, ensure homogeneous cell seeding, and promote cell migration. As a proof-of-concept demonstration, we have created skeletal muscle patches with a thickness of approximately 150 μm using SHAIPs. The myoblasts seeded in the scaffolds maintained high viability and were able to differentiate into multi-nucleated myotubes. Moreover, neovasculature could efficiently develop into the patches upon subcutaneous implantation in vivo.

Full Text

Duke Authors

Cited Authors

  • Zhang, YS; Yao, J; Wang, LV; Xia, Y

Published Date

  • January 2014

Published In

Volume / Issue

  • 55 / 1

Start / End Page

  • 445 - 452

PubMed ID

  • 24443593

Pubmed Central ID

  • 24443593

Electronic International Standard Serial Number (EISSN)

  • 1873-2291

International Standard Serial Number (ISSN)

  • 0032-3861

Digital Object Identifier (DOI)

  • 10.1016/j.polymer.2013.06.019


  • eng