Photoacoustic Microscopy.

Published

Journal Article

Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.

Full Text

Duke Authors

Cited Authors

  • Yao, J; Wang, LV

Published Date

  • September 2013

Published In

Volume / Issue

  • 7 / 5

PubMed ID

  • 24416085

Pubmed Central ID

  • 24416085

Electronic International Standard Serial Number (EISSN)

  • 1863-8899

International Standard Serial Number (ISSN)

  • 1863-8880

Digital Object Identifier (DOI)

  • 10.1002/lpor.201200060

Language

  • eng