Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo.

Journal Article (Journal Article)

We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which diffuses well across the blood-brain-barrier, provided exogenous contrast for photoacoustic imaging of glucose response. Concurrently, hemoglobin provided endogenous contrast for photoacoustic imaging of hemodynamic response. Glucose and hemodynamic responses were quantitatively decoupled by using two-wavelength measurements. We found that glucose uptake and blood perfusion around the somatosensory region of the contralateral hemisphere were both increased by stimulations, indicating elevated neuron activity. While the glucose response area was more homogenous and confined within the somatosensory region, the hemodynamic response area had a clear vascular pattern and spread wider than the somatosensory region. Our results demonstrate that 2-NBDG-enhanced PACT is a promising tool for noninvasive studies of brain metabolism.

Full Text

Duke Authors

Cited Authors

  • Yao, J; Xia, J; Maslov, KI; Nasiriavanaki, M; Tsytsarev, V; Demchenko, AV; Wang, LV

Published Date

  • January 2013

Published In

Volume / Issue

  • 64 /

Start / End Page

  • 257 - 266

PubMed ID

  • 22940116

Pubmed Central ID

  • PMC3508393

Electronic International Standard Serial Number (EISSN)

  • 1095-9572

International Standard Serial Number (ISSN)

  • 1053-8119

Digital Object Identifier (DOI)

  • 10.1016/j.neuroimage.2012.08.054


  • eng