Double-illumination photoacoustic microscopy.

Journal Article (Journal Article)

Recent developments of optical-resolution photoacoustic microscopy (OR-PAM) have improved its spatial resolution and imaging speed. However, the penetration depth of OR-PAM is still limited to ~1 mm in tissue, owing to the strong tissue scattering. Here, we have developed double-illumination PAM (DI-PAM), which illuminates the sample from both top and bottom sides simultaneously. Through phantom and in vivo experiments, we have demonstrated for thin targets that DI-PAM has a penetration depth of ~2 mm in tissue at 532 nm and a focal zone of 260 μm, both significant improvements over traditional reflection or transmission-mode OR-PAM.

Full Text

Duke Authors

Cited Authors

  • Yao, J; Maslov, KI; Puckett, ER; Rowland, KJ; Warner, BW; Wang, LV

Published Date

  • February 2012

Published In

Volume / Issue

  • 37 / 4

Start / End Page

  • 659 - 661

PubMed ID

  • 22344139

Pubmed Central ID

  • PMC3306616

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.37.000659


  • eng