Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo.
Published
Journal Article
Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks--especially capillaries--are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated.
Full Text
Duke Authors
Cited Authors
- Yao, J; Maslov, K; Hu, S; Wang, LV
Published Date
- September 2009
Published In
Volume / Issue
- 14 / 5
Start / End Page
- 054049 -
PubMed ID
- 19895150
Pubmed Central ID
- 19895150
Electronic International Standard Serial Number (EISSN)
- 1560-2281
International Standard Serial Number (ISSN)
- 1083-3668
Digital Object Identifier (DOI)
- 10.1117/1.3251044
Language
- eng