Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo.

Journal Article (Journal Article)

Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks--especially capillaries--are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated.

Full Text

Duke Authors

Cited Authors

  • Yao, J; Maslov, K; Hu, S; Wang, LV

Published Date

  • September 2009

Published In

Volume / Issue

  • 14 / 5

Start / End Page

  • 054049 -

PubMed ID

  • 19895150

Pubmed Central ID

  • PMC2782364

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.3251044


  • eng