Photoacoustic microscopy: superdepth, superresolution, and superb contrast.

Journal Article

Since its invention in the 17th century, optical microscopy has revolutionized biomedical studies by scrutinizing the biological realm on cellular levels, taking advantage of its excellent light-focusing capability. However, most biological tissues scatter light highly. As light travels in tissue, cumulative scattering events cause the photons to lose their original propagation direction and, thus, their ability to be focused, which has largely limited the penetration depth of optical microscopy. Conventional planar optical microscopy can provide penetration of only ~100 ?m before photons begin to be scattered. The penetration of modern optical microcopy, such as confocal microscopy and multiphoton microscopy, is still limited to approximately the optical diffusion limit (~1 mm in the skin as approximated by one optical transport mean free path), where scattered photons retain a strong memory of the original propagation direction. So far, it still remains a challenge for pure optical methods to achieve high-resolution in vivo imaging beyond the diffusion limit (i.e., superdepth imaging).

Full Text

Duke Authors

Cited Authors

  • Yao, J; Song, L; Wang, LV

Published Date

  • May 2015

Published In

Volume / Issue

  • 6 / 3

Start / End Page

  • 34 - 37

PubMed ID

  • 25974913

Pubmed Central ID

  • 25974913

Electronic International Standard Serial Number (EISSN)

  • 2154-2317

International Standard Serial Number (ISSN)

  • 2154-2287

Digital Object Identifier (DOI)

  • 10.1109/mpul.2015.2409100


  • eng