Photoacoustic microscopy: superdepth, superresolution, and superb contrast.
Journal Article
Since its invention in the 17th century, optical microscopy has revolutionized biomedical studies by scrutinizing the biological realm on cellular levels, taking advantage of its excellent light-focusing capability. However, most biological tissues scatter light highly. As light travels in tissue, cumulative scattering events cause the photons to lose their original propagation direction and, thus, their ability to be focused, which has largely limited the penetration depth of optical microscopy. Conventional planar optical microscopy can provide penetration of only ~100 ?m before photons begin to be scattered. The penetration of modern optical microcopy, such as confocal microscopy and multiphoton microscopy, is still limited to approximately the optical diffusion limit (~1 mm in the skin as approximated by one optical transport mean free path), where scattered photons retain a strong memory of the original propagation direction. So far, it still remains a challenge for pure optical methods to achieve high-resolution in vivo imaging beyond the diffusion limit (i.e., superdepth imaging).
Full Text
Duke Authors
Cited Authors
- Yao, J; Song, L; Wang, LV
Published Date
- May 2015
Published In
Volume / Issue
- 6 / 3
Start / End Page
- 34 - 37
PubMed ID
- 25974913
Pubmed Central ID
- 25974913
Electronic International Standard Serial Number (EISSN)
- 2154-2317
International Standard Serial Number (ISSN)
- 2154-2287
Digital Object Identifier (DOI)
- 10.1109/mpul.2015.2409100
Language
- eng