Photoacoustic imaging of single circulating melanoma cells in vivo

Published

Conference Paper

© 2015 SPIE. Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.

Full Text

Duke Authors

Cited Authors

  • Wang, L; Yao, J; Zhang, R; Xu, S; Li, G; Zou, J; Wang, LV

Published Date

  • January 1, 2015

Published In

Volume / Issue

  • 9323 /

International Standard Serial Number (ISSN)

  • 1605-7422

International Standard Book Number 13 (ISBN-13)

  • 9781628414134

Digital Object Identifier (DOI)

  • 10.1117/12.2076987

Citation Source

  • Scopus