Photo-imprint super-resolution photoacoustic microscopy

Published

Conference Paper

© 2015 SPIE. Combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for super-resolution photoacoustic imaging of both fluorescent and non-fluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. By scanning the excitation beam, we performed three-dimensional sub-diffraction imaging of varied fluorescent and non-fluorescent species. A lateral resolution of 80 nm and an axial resolution of 370 nm have been demonstrated. This technique has the potential to enable label-free super-resolution imaging, and can be transferred to other optical imaging modalities or combined with other super-resolution methods.

Full Text

Duke Authors

Cited Authors

  • Yao, J; Wang, L; Li, C; Zhang, C; Wang, LV

Published Date

  • January 1, 2015

Published In

Volume / Issue

  • 9323 /

International Standard Serial Number (ISSN)

  • 1605-7422

International Standard Book Number 13 (ISBN-13)

  • 9781628414134

Digital Object Identifier (DOI)

  • 10.1117/12.2077350

Citation Source

  • Scopus