Skip to main content
Journal cover image

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Publication ,  Conference
Yang, JM; Li, C; Chen, R; Rao, B; Yao, J; Yeh, CH; Danielli, A; Maslov, K; Zhou, Q; Shung, KK; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
March 11, 2015

Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ∼10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414134

Publication Date

March 11, 2015

Volume

9323
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yang, J. M., Li, C., Chen, R., Rao, B., Yao, J., Yeh, C. H., … Wang, L. V. (2015). Label-free optical-resolution photoacoustic endomicroscopy in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9323). https://doi.org/10.1117/12.2080224
Yang, J. M., C. Li, R. Chen, B. Rao, J. Yao, C. H. Yeh, A. Danielli, et al. “Label-free optical-resolution photoacoustic endomicroscopy in vivo.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 9323, 2015. https://doi.org/10.1117/12.2080224.
Yang JM, Li C, Chen R, Rao B, Yao J, Yeh CH, et al. Label-free optical-resolution photoacoustic endomicroscopy in vivo. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Yang, J. M., et al. “Label-free optical-resolution photoacoustic endomicroscopy in vivo.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9323, 2015. Scopus, doi:10.1117/12.2080224.
Yang JM, Li C, Chen R, Rao B, Yao J, Yeh CH, Danielli A, Maslov K, Zhou Q, Shung KK, Wang LV. Label-free optical-resolution photoacoustic endomicroscopy in vivo. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2015.
Journal cover image

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9781628414134

Publication Date

March 11, 2015

Volume

9323