Label-free optical-resolution photoacoustic endomicroscopy in vivo


Conference Paper

© 2015 SPIE. Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ∼10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.

Full Text

Duke Authors

Cited Authors

  • Yang, JM; Li, C; Chen, R; Rao, B; Yao, J; Yeh, CH; Danielli, A; Maslov, K; Zhou, Q; Shung, KK; Wang, LV

Published Date

  • January 1, 2015

Published In

Volume / Issue

  • 9323 /

International Standard Serial Number (ISSN)

  • 1605-7422

International Standard Book Number 13 (ISBN-13)

  • 9781628414134

Digital Object Identifier (DOI)

  • 10.1117/12.2080224

Citation Source

  • Scopus