Near-infrared optical-resolution photoacoustic microscopy.

Journal Article (Journal Article)

Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100  mJ/cm2). Beyond ∼0.6  mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.

Full Text

Duke Authors

Cited Authors

  • Hai, P; Yao, J; Maslov, KI; Zhou, Y; Wang, LV

Published Date

  • September 2014

Published In

Volume / Issue

  • 39 / 17

Start / End Page

  • 5192 - 5195

PubMed ID

  • 25166107

Pubmed Central ID

  • PMC4161671

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.39.005192


  • eng