Near-infrared optical-resolution photoacoustic microscopy.
Journal Article (Journal Article)
Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
Full Text
Duke Authors
Cited Authors
- Hai, P; Yao, J; Maslov, KI; Zhou, Y; Wang, LV
Published Date
- September 2014
Published In
Volume / Issue
- 39 / 17
Start / End Page
- 5192 - 5195
PubMed ID
- 25166107
Pubmed Central ID
- PMC4161671
Electronic International Standard Serial Number (EISSN)
- 1539-4794
International Standard Serial Number (ISSN)
- 0146-9592
Digital Object Identifier (DOI)
- 10.1364/ol.39.005192
Language
- eng