Optical-resolution photoacoustic microscopy for volumetric and spectral analysis of histological and immunochemical samples.


Journal Article

Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.

Full Text

Duke Authors

Cited Authors

  • Zhang, YS; Yao, J; Zhang, C; Li, L; Wang, LV; Xia, Y

Published Date

  • July 2014

Published In

Volume / Issue

  • 53 / 31

Start / End Page

  • 8099 - 8103

PubMed ID

  • 24961608

Pubmed Central ID

  • 24961608

Electronic International Standard Serial Number (EISSN)

  • 1521-3773

International Standard Serial Number (ISSN)

  • 1433-7851

Digital Object Identifier (DOI)

  • 10.1002/anie.201403812


  • eng