Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds


Conference Paper

It is a challenge to non-invasively visualize in vivo the neovascularization in a three-dimensional (3D) scaffold with high spatial resolution and deep penetration depth. Here we used photoacoustic microscopy (PAM) to chronically monitor neovascularization in an inverse opal scaffold implanted in a mouse model for up to six weeks. The neovasculature was observed to develop gradually in the same mouse. These blood vessels not only grew on top of the implanted scaffold but also penetrated into the scaffold. The PAM system offered a lateral resolution of ∼45 μm and a penetration depth of ∼3 mm into the scaffold/tissue construct. By using the 3D PAM data, we further quantified the vessel area as a function of time. © 2013 Copyright SPIE.

Full Text

Duke Authors

Cited Authors

  • Cai, X; Zhang, Y; Li, L; Choi, SW; MacEwan, MR; Yao, J; Kim, C; Xia, Y; Wang, LV

Published Date

  • May 28, 2013

Published In

Volume / Issue

  • 8581 /

International Standard Serial Number (ISSN)

  • 1605-7422

International Standard Book Number 13 (ISBN-13)

  • 9780819493507

Digital Object Identifier (DOI)

  • 10.1117/12.2005236

Citation Source

  • Scopus