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Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection

Publication ,  Conference
Yao, J; Rowland, KJ; Wang, L; Maslov, KI; Warner, BW; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
February 23, 2012

Massive small bowel resection (SBR) results in villus angiogenesis and intestinal adaptation. The exact mechanism that causes intestinal villus angiogenesis remains unknown. We hypothesize that hemodynamic changes within the remnant bowel after SBR will trigger intestinal angiogenesis. To validate this, we used photoacoustic microscopy (PAM) to image the microvascular system of the intestine in C57B6 mice and to measure blood flow and oxygen saturation (sO2) of a supplying artery and vein. Baseline measurements were made 6 cm proximal to the ileal-cecal junction (ICJ) prior to resection. A 50% proximal bowel resection was then performed, and measurements were again recorded at the same location immediately, 1, 3 and 7 days following resection. The results show that arterial and venous sO2 were similar prior to SBR. Immediately following SBR, the arterial and venous sO2 decreased by 14.3 ± 2.7% and 32.7 ± 6.6%, respectively, while the arterial and venous flow speed decreased by 62.9 ± 17.3% and 60.0 ± 20.1%, respectively. Such significant decreases in sO2 and blood flow indicate a hypoxic state after SBR. Within one week after SBR, both sO 2 and blood flow speed had gradually recovered. By 7 days after SBR, arterial and venous sO2 had increased to 101.0 ± 2.9% and 82.7 ± 7.3% of the baseline values, respectively, while arterial and venous flow speed had increased to 106.0 ± 21.4% and 150.0 ± 29.6% of the baseline values, respectively. Such increases in sO2 and blood flow may result from angiogenesis following SBR. © 2012 SPIE.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819488664

Publication Date

February 23, 2012

Volume

8223
 

Citation

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Yao, J., Rowland, K. J., Wang, L., Maslov, K. I., Warner, B. W., & Wang, L. V. (2012). Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8223). https://doi.org/10.1117/12.909508
Yao, J., K. J. Rowland, L. Wang, K. I. Maslov, B. W. Warner, and L. V. Wang. “Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 8223, 2012. https://doi.org/10.1117/12.909508.
Yao J, Rowland KJ, Wang L, Maslov KI, Warner BW, Wang LV. Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2012.
Yao, J., et al. “Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8223, 2012. Scopus, doi:10.1117/12.909508.
Yao J, Rowland KJ, Wang L, Maslov KI, Warner BW, Wang LV. Double-illumination photoacoustic microscopy of intestinal hemodynamics following massive small bowel resection. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2012.
Journal cover image

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819488664

Publication Date

February 23, 2012

Volume

8223