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Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo

Publication ,  Conference
Krumholz, A; Filonov, GS; Xia, J; Yao, J; Verkhusha, VV; Wang, LV
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
February 9, 2012

Genetically encoded probes powerfully and non-invasively target specific tissues, cells, and subcellular locations. iRFP, a novel near-infrared fluorescent protein with low quantum yield whose absorption and fluorescence maxima are located at wavelengths longer than the Q-band of hemoglobin absorption, is ideal for PAT. Here, we report on an in vitro comparison of iRFP with other far-red fluorescent proteins, and its use in imaging a mouse tumor xenograft model. In an in vivo experiment, we stably transfected iRFP into MTLn3 adenocarcinoma cells and injected them into the mammary fat pad of female SCID/NCr mice, then imaged the resulting tumors two and three weeks post injection. The contrast increase from the protein expression was high enough to clearly separate the tumor region from the rest of the animal. © 2012 SPIE.

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819488664

Publication Date

February 9, 2012

Volume

8223
 

Citation

APA
Chicago
ICMJE
MLA
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Krumholz, A., Filonov, G. S., Xia, J., Yao, J., Verkhusha, V. V., & Wang, L. V. (2012). Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8223). https://doi.org/10.1117/12.908927
Krumholz, A., G. S. Filonov, J. Xia, J. Yao, V. V. Verkhusha, and L. V. Wang. “Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo.” In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 8223, 2012. https://doi.org/10.1117/12.908927.
Krumholz A, Filonov GS, Xia J, Yao J, Verkhusha VV, Wang LV. Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2012.
Krumholz, A., et al. “Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8223, 2012. Scopus, doi:10.1117/12.908927.
Krumholz A, Filonov GS, Xia J, Yao J, Verkhusha VV, Wang LV. Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2012.
Journal cover image

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

ISBN

9780819488664

Publication Date

February 9, 2012

Volume

8223