Photoacoustic microscopy of tyrosinase reporter gene in vivo.

Journal Article (Letter)

Photoacoustic tomography is a hybrid modality based on optical absorption excitation and ultrasonic detection. It is sensitive to melanin, one of the primary absorbers in skin. For cells that do not naturally contain melanin, melanin production can be induced by introducing the gene for tyrosinase, the primary enzyme responsible for expression of melanin in melanogenic cells. Optical resolution photoacoustic microscopy was used in the ex vivo study reported here, where the signal from transfected cells increased by more than 10 times over wild-type cells. A subsequent in vivo experiment was conducted to demonstrate the capability of photoacoustic microscopy to spectrally differentiate between tyrosinase-catalyzed melanin and various other absorbers in tissue.

Full Text

Duke Authors

Cited Authors

  • Krumholz, A; Vanvickle-Chavez, SJ; Yao, J; Fleming, TP; Gillanders, WE; Wang, LV

Published Date

  • August 2011

Published In

Volume / Issue

  • 16 / 8

Start / End Page

  • 080503 -

PubMed ID

  • 21895303

Pubmed Central ID

  • PMC3162617

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.3606568


  • eng