Carbohydrate Microarrays Identify Blood Group Precursor Cryptic Epitopes as Potential Immunological Targets of Breast Cancer.

Journal Article (Journal Article)

Using carbohydrate microarrays, we explored potential natural ligands of antitumor monoclonal antibody HAE3. This antibody was raised against a murine mammary tumor antigen but was found to cross-react with a number of human epithelial tumors in tissues. Our carbohydrate microarray analysis reveals that HAE3 is specific for an O-glycan cryptic epitope that is normally hidden in the cores of blood group substances. Using HAE3 to screen tumor cell surface markers by flow cytometry, we found that the HAE3 glycoepitope, gp(HAE3), was highly expressed by a number of human breast cancer cell lines, including some triple-negative cancers that lack the estrogen, progesterone, and Her2/neu receptors. Taken together, we demonstrate that HAE3 recognizes a conserved cryptic glycoepitope of blood group precursors, which is nevertheless selectively expressed and surface-exposed in certain breast tumor cells. The potential of this class of O-glycan cryptic antigens in breast cancer subtyping and targeted immunotherapy warrants further investigation.

Full Text

Duke Authors

Cited Authors

  • Wang, D; Tang, J; Liu, S; Huang, J

Published Date

  • 2015

Published In

Volume / Issue

  • 2015 /

Start / End Page

  • 510810 -

PubMed ID

  • 26539555

Pubmed Central ID

  • PMC4619957

Electronic International Standard Serial Number (EISSN)

  • 2314-7156

Digital Object Identifier (DOI)

  • 10.1155/2015/510810

Language

  • eng

Conference Location

  • Egypt