Inhibition of microRNA 128 promotes excitability of cultured cortical neuronal networks.


Journal Article

Cultured neuronal networks monitored with microelectrode arrays (MEAs) have been used widely to evaluate pharmaceutical compounds for potential neurotoxic effects. A newer application of MEAs has been in the development of in vitro models of neurological disease. Here, we directly evaluated the utility of MEAs to recapitulate in vivo phenotypes of mature microRNA-128 (miR-128) deficiency, which causes fatal seizures in mice. We show that inhibition of miR-128 results in significantly increased neuronal activity in cultured neuronal networks derived from primary mouse cortical neurons. These results support the utility of MEAs in developing in vitro models of neuroexcitability disorders, such as epilepsy, and further suggest that MEAs provide an effective tool for the rapid identification of microRNAs that promote seizures when dysregulated.

Full Text

Cited Authors

  • McSweeney, KM; Gussow, AB; Bradrick, SS; Dugger, SA; Gelfman, S; Wang, Q; Petrovski, S; Frankel, WN; Boland, MJ; Goldstein, DB

Published Date

  • October 2016

Published In

Volume / Issue

  • 26 / 10

Start / End Page

  • 1411 - 1416

PubMed ID

  • 27516621

Pubmed Central ID

  • 27516621

Electronic International Standard Serial Number (EISSN)

  • 1549-5469

International Standard Serial Number (ISSN)

  • 1088-9051

Digital Object Identifier (DOI)

  • 10.1101/gr.199828.115


  • eng