Carbon extension in peptidylnucleoside biosynthesis by radical SAM enzymes.

Journal Article (Journal Article)

Nikkomycins and polyoxins are antifungal peptidylnucleoside antibiotics active against human and plant pathogens. Here we report that during peptidylnucleoside biosynthesis in Streptomyces cacaoi and S. tendae, the C5' extension of the nucleoside essential for downstream structural diversification is catalyzed by a conserved radical S-adenosyl-L-methionine (SAM) enzyme, PolH or NikJ. This is distinct from the nucleophilic mechanism reported for antibacterial nucleosides and represents a new mechanism of nucleoside natural product biosynthesis.

Full Text

Duke Authors

Cited Authors

  • Lilla, EA; Yokoyama, K

Published Date

  • November 2016

Published In

Volume / Issue

  • 12 / 11

Start / End Page

  • 905 - 907

PubMed ID

  • 27642865

Pubmed Central ID

  • PMC5069167

Electronic International Standard Serial Number (EISSN)

  • 1552-4469

Digital Object Identifier (DOI)

  • 10.1038/nchembio.2187


  • eng

Conference Location

  • United States