Transcriptional Timers Regulating Mitosis in Early Drosophila Embryos.

Published

Journal Article

The development of an embryo requires precise spatiotemporal regulation of cellular processes. During Drosophila gastrulation, a precise temporal pattern of cell division is encoded through transcriptional regulation of cdc25(string) in 25 distinct mitotic domains. Using a genetic screen, we demonstrate that the same transcription factors that regulate the spatial pattern of cdc25(string) transcription encode its temporal activation. We identify buttonhead and empty spiracles as the major activators of cdc25(string) expression in mitotic domain 2. The effect of these activators is balanced through repression by hairy, sloppy paired 1, and huckebein. Within the mitotic domain, temporal precision of mitosis is robust and unaffected by changing dosage of rate-limiting transcriptional factors. However, precision can be disrupted by altering the levels of the two activators or two repressors. We propose that the additive and balanced action of activators and repressors is a general strategy for precise temporal regulation of cellular transitions during development.

Full Text

Duke Authors

Cited Authors

  • Momen-Roknabadi, A; Di Talia, S; Wieschaus, E

Published Date

  • September 13, 2016

Published In

Volume / Issue

  • 16 / 11

Start / End Page

  • 2793 - 2801

PubMed ID

  • 27626650

Pubmed Central ID

  • 27626650

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2016.08.034

Language

  • eng

Conference Location

  • United States