Pentraxin 3 recruits complement factor H to protect against oxidative stress-induced complement and inflammasome overactivation.

Published

Journal Article

The discovery that genetic abnormalities in complement factor H (FH) are associated with an increased risk for age-related macular degeneration (AMD), the most common cause of blindness among the elderly, raised hope of new treatments for this vision-threatening disease. Nonetheless, over a decade after the identification of this important association, how innate immunity contributes to AMD remains unresolved. Pentraxin 3 (PTX3), an essential component of the innate immunity system that plays a non-redundant role in controlling inflammation, regulates complement by interacting with complement components. Here, we show that PTX3 is induced by oxidative stress, a known cause of AMD, in the retinal pigmented epithelium (RPE). PTX3 deficiency in vitro and in vivo magnified complement activation induced by oxidative stress, leading to increased C3a, FB, and C3d, but not C5b-9 complex formation. Increased C3a levels, resulting from PTX3 deficiency, raised the levels of Il1b mRNA and secretion of activated interleukin (IL)-1β by interacting with C3aR. Importantly, PTX3 deficiency augmented NLRP3 inflammasome activation, resulting in enhanced IL-1β, but not IL-18, production by the RPE. Thus, in the presence of PTX3 deficiency, the complement and inflammasome pathways worked in concert to produce IL-1β in sufficient abundance to, importantly, result in macrophages accumulating in the choroid. These results demonstrate that PTX3 acts as an essential brake for complement and inflammasome activation by regulating the abundance of FH in the RPE, and provide critical insights into the complex interplay between oxidative stress and innate immunity in the early stages of AMD development. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Full Text

Cited Authors

  • Wang, L; Cano, M; Datta, S; Wei, H; Ebrahimi, KB; Gorashi, Y; Garlanda, C; Handa, JT

Published Date

  • December 2016

Published In

Volume / Issue

  • 240 / 4

Start / End Page

  • 495 - 506

PubMed ID

  • 27659908

Pubmed Central ID

  • 27659908

Electronic International Standard Serial Number (EISSN)

  • 1096-9896

International Standard Serial Number (ISSN)

  • 0022-3417

Digital Object Identifier (DOI)

  • 10.1002/path.4811

Language

  • eng