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Transcription-associated mutation of lasR in Pseudomonas aeruginosa.

Publication ,  Journal Article
Wang, C; McPherson, JR; Zhang, L-H; Rozen, S; Sabapathy, K
Published in: DNA Repair (Amst)
October 2016

Pseudomonas aeruginosa is an opportunistic pathogen which infects cystic fibrosis and cancer patients with compromised immune systems. LasR is a master regulator which controls the virulence of P. aeruginosa in response to bacterial cell-density and host signals. During infection, lasR is frequently mutated, conferring P. aeruginosa a growth advantage in hosts and enhances resistance to widely used antibiotics. However, the mechanistic basis of lasR mutation is not well understood. We have tested here the hypothesis that transcription strength is a contributory determinant of lasR mutagenesis. P. aeruginosa strains with different lasR transcription strengths were therefore engineered and the lasR mutations were monitored unbiasedly using next-generation sequencing technology. Our results suggest that the strength of transcription could be one of the deterministic factors that drive the mutagenesis of lasR in P. aeruginosa, shedding new insights into bacterial infection and antibiotic resistance.

Duke Scholars

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Published In

DNA Repair (Amst)

DOI

EISSN

1568-7856

Publication Date

October 2016

Volume

46

Start / End Page

9 / 19

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Trans-Activators
  • Repressor Proteins
  • Quorum Sensing
  • Pseudomonas aeruginosa
  • Plasmids
  • Mutation
  • High-Throughput Nucleotide Sequencing
  • Genetic Fitness
  • Gene Expression Regulation, Bacterial
 

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Wang, C., McPherson, J. R., Zhang, L.-H., Rozen, S., & Sabapathy, K. (2016). Transcription-associated mutation of lasR in Pseudomonas aeruginosa. DNA Repair (Amst), 46, 9–19. https://doi.org/10.1016/j.dnarep.2016.09.001
Wang, Chao, John R. McPherson, Lian-Hui Zhang, Steve Rozen, and Kanaga Sabapathy. “Transcription-associated mutation of lasR in Pseudomonas aeruginosa.DNA Repair (Amst) 46 (October 2016): 9–19. https://doi.org/10.1016/j.dnarep.2016.09.001.
Wang C, McPherson JR, Zhang L-H, Rozen S, Sabapathy K. Transcription-associated mutation of lasR in Pseudomonas aeruginosa. DNA Repair (Amst). 2016 Oct;46:9–19.
Wang, Chao, et al. “Transcription-associated mutation of lasR in Pseudomonas aeruginosa.DNA Repair (Amst), vol. 46, Oct. 2016, pp. 9–19. Pubmed, doi:10.1016/j.dnarep.2016.09.001.
Wang C, McPherson JR, Zhang L-H, Rozen S, Sabapathy K. Transcription-associated mutation of lasR in Pseudomonas aeruginosa. DNA Repair (Amst). 2016 Oct;46:9–19.
Journal cover image

Published In

DNA Repair (Amst)

DOI

EISSN

1568-7856

Publication Date

October 2016

Volume

46

Start / End Page

9 / 19

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Trans-Activators
  • Repressor Proteins
  • Quorum Sensing
  • Pseudomonas aeruginosa
  • Plasmids
  • Mutation
  • High-Throughput Nucleotide Sequencing
  • Genetic Fitness
  • Gene Expression Regulation, Bacterial