Transcription-associated mutation of lasR in Pseudomonas aeruginosa.

Journal Article (Journal Article)

Pseudomonas aeruginosa is an opportunistic pathogen which infects cystic fibrosis and cancer patients with compromised immune systems. LasR is a master regulator which controls the virulence of P. aeruginosa in response to bacterial cell-density and host signals. During infection, lasR is frequently mutated, conferring P. aeruginosa a growth advantage in hosts and enhances resistance to widely used antibiotics. However, the mechanistic basis of lasR mutation is not well understood. We have tested here the hypothesis that transcription strength is a contributory determinant of lasR mutagenesis. P. aeruginosa strains with different lasR transcription strengths were therefore engineered and the lasR mutations were monitored unbiasedly using next-generation sequencing technology. Our results suggest that the strength of transcription could be one of the deterministic factors that drive the mutagenesis of lasR in P. aeruginosa, shedding new insights into bacterial infection and antibiotic resistance.

Full Text

Duke Authors

Cited Authors

  • Wang, C; McPherson, JR; Zhang, L-H; Rozen, S; Sabapathy, K

Published Date

  • October 2016

Published In

Volume / Issue

  • 46 /

Start / End Page

  • 9 - 19

PubMed ID

  • 27650847

Electronic International Standard Serial Number (EISSN)

  • 1568-7856

Digital Object Identifier (DOI)

  • 10.1016/j.dnarep.2016.09.001


  • eng

Conference Location

  • Netherlands