Skip to main content
Journal cover image

Plasma acylcarnitines during insulin stimulation in humans are reflective of age-related metabolic dysfunction.

Publication ,  Journal Article
Consitt, LA; Koves, TR; Muoio, DM; Nakazawa, M; Newton, CA; Houmard, JA
Published in: Biochem Biophys Res Commun
October 28, 2016

The purpose of this study was to determine if plasma acylcarnitine (AC) profiling is altered under hyperinsulinemic conditions as part of the aging process. Fifteen young, lean (19-29 years) and fifteen middle-to older-aged (57-82 years) individuals underwent a 2-hr euglycemic-hyperinsulinemic clamp. Plasma samples were obtained at baseline, 20 min, 50 min, and 120 min for analysis of AC species and amino acids. Skeletal muscle biopsies were performed after 60 min of insulin-stimulation for analysis of acetyl-CoA carboxylase (ACC) phosphorylation. Insulin infusion decreased the majority of plasma short-, medium-, and long-chain (SC, MC, and LC, respectively) AC. However, during the initial 50 min, a number of MC and LC AC species (C10, C10:1, C12:1, C14, C16, C16:1, C18) remained elevated in aged individuals compared to their younger counterparts indicating a lag in responsiveness. Additionally, the insulin-induced decline in skeletal muscle ACC phosphorylation was blunted in the aged compared to young individuals (-24% vs. -56%, P < 0.05). These data suggest that a desensitization to insulin during aging, possibly at the level of skeletal muscle ACC phosphorylation, results in a diminished ability to transition to glucose oxidation indicative of metabolic inflexibility.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

October 28, 2016

Volume

479

Issue

4

Start / End Page

868 / 874

Location

United States

Related Subject Headings

  • Young Adult
  • Phosphorylation
  • Oxidation-Reduction
  • Muscle, Skeletal
  • Middle Aged
  • Male
  • Insulin Resistance
  • Insulin
  • Humans
  • Glucose Clamp Technique
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Consitt, L. A., Koves, T. R., Muoio, D. M., Nakazawa, M., Newton, C. A., & Houmard, J. A. (2016). Plasma acylcarnitines during insulin stimulation in humans are reflective of age-related metabolic dysfunction. Biochem Biophys Res Commun, 479(4), 868–874. https://doi.org/10.1016/j.bbrc.2016.09.116
Consitt, Leslie A., Timothy R. Koves, Deborah M. Muoio, Masato Nakazawa, Christopher A. Newton, and Joseph A. Houmard. “Plasma acylcarnitines during insulin stimulation in humans are reflective of age-related metabolic dysfunction.Biochem Biophys Res Commun 479, no. 4 (October 28, 2016): 868–74. https://doi.org/10.1016/j.bbrc.2016.09.116.
Consitt LA, Koves TR, Muoio DM, Nakazawa M, Newton CA, Houmard JA. Plasma acylcarnitines during insulin stimulation in humans are reflective of age-related metabolic dysfunction. Biochem Biophys Res Commun. 2016 Oct 28;479(4):868–74.
Consitt, Leslie A., et al. “Plasma acylcarnitines during insulin stimulation in humans are reflective of age-related metabolic dysfunction.Biochem Biophys Res Commun, vol. 479, no. 4, Oct. 2016, pp. 868–74. Pubmed, doi:10.1016/j.bbrc.2016.09.116.
Consitt LA, Koves TR, Muoio DM, Nakazawa M, Newton CA, Houmard JA. Plasma acylcarnitines during insulin stimulation in humans are reflective of age-related metabolic dysfunction. Biochem Biophys Res Commun. 2016 Oct 28;479(4):868–874.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

October 28, 2016

Volume

479

Issue

4

Start / End Page

868 / 874

Location

United States

Related Subject Headings

  • Young Adult
  • Phosphorylation
  • Oxidation-Reduction
  • Muscle, Skeletal
  • Middle Aged
  • Male
  • Insulin Resistance
  • Insulin
  • Humans
  • Glucose Clamp Technique