Control of phenylalanine hydroxylase synthesis in tissue culture by serum and insulin


Journal Article

Stationary‐phase, minimal deviation hepatoma H4‐II‐E‐C3 cell cultures that are serum‐deprived respond with a biphasic time course of phenyl‐alanine hydroxylase induction when dialyzed fetal calf serum or insulin is added. These two agents induce phenylalanine hydroxylase additively, during both the initial 3‐hour and the delayed 24‐hour phases. The initial phase of induction by insulin is inhibited by cycloheximide but not by actinomycin D. The delayed induction by both dialyzed fetal calf serum and insulin is inhibited by 10−6 M cycloheximide and 0.20 μg/ml actinomycin D. H4‐II‐E‐C3 cells in culture do not synthesize the factor(s) in serum that induce phenylalanine hydroxylase. Copyright © 1976 Wiley‐Liss, Inc.

Full Text

Duke Authors

Cited Authors

  • Tourian, A

Published Date

  • January 1, 1976

Published In

Volume / Issue

  • 87 / 1

Start / End Page

  • 15 - 23

PubMed ID

  • 1401

Pubmed Central ID

  • 1401

Electronic International Standard Serial Number (EISSN)

  • 1097-4652

International Standard Serial Number (ISSN)

  • 0021-9541

Digital Object Identifier (DOI)

  • 10.1002/jcp.1040870104

Citation Source

  • Scopus