Control of phenylalanine hydroxylase synthesis in tissue culture by serum and insulin.
Publication
, Journal Article
Tourian, A
Published in: J Cell Physiol
January 1976
Stationary-phase, minimal deviation hepatoma H4-II-E-C3 cell cultures that are serum-deprived respond with a biphasic time course of phenylalanine hydroxylase induction when dialyzed fetal calf serum or insulin is added. These two agents induce phenylalanine hydroxylase additively, during both the initial 3-hour and the delayed 24-hour phases. The initial phase of induction by insulin is inhibited by cycloheximide but not by actinomycin D. The delayed induction by both dialyzed fetal calf serum and insulin is inhibited by 10(-6) M cycloheximide and 0.20 mug/ml actinomycin D. H4-II-E-C3 cells in culture do not synthesize the factor(s) in serum that induce phenylalanine hydroxylase.
Duke Scholars
Published In
J Cell Physiol
DOI
ISSN
0021-9541
Publication Date
January 1976
Volume
87
Issue
1
Start / End Page
15 / 24
Location
United States
Related Subject Headings
- Uridine
- Ultrafiltration
- Tyrosine Transaminase
- Thymidine
- RNA
- Protein Biosynthesis
- Phenylalanine Hydroxylase
- Kinetics
- Insulin
- Enzyme Induction
Citation
APA
Chicago
ICMJE
MLA
NLM
Tourian, A. (1976). Control of phenylalanine hydroxylase synthesis in tissue culture by serum and insulin. J Cell Physiol, 87(1), 15–24. https://doi.org/10.1002/jcp.1040870104
Tourian, A. “Control of phenylalanine hydroxylase synthesis in tissue culture by serum and insulin.” J Cell Physiol 87, no. 1 (January 1976): 15–24. https://doi.org/10.1002/jcp.1040870104.
Tourian A. Control of phenylalanine hydroxylase synthesis in tissue culture by serum and insulin. J Cell Physiol. 1976 Jan;87(1):15–24.
Tourian, A. “Control of phenylalanine hydroxylase synthesis in tissue culture by serum and insulin.” J Cell Physiol, vol. 87, no. 1, Jan. 1976, pp. 15–24. Pubmed, doi:10.1002/jcp.1040870104.
Tourian A. Control of phenylalanine hydroxylase synthesis in tissue culture by serum and insulin. J Cell Physiol. 1976 Jan;87(1):15–24.
Published In
J Cell Physiol
DOI
ISSN
0021-9541
Publication Date
January 1976
Volume
87
Issue
1
Start / End Page
15 / 24
Location
United States
Related Subject Headings
- Uridine
- Ultrafiltration
- Tyrosine Transaminase
- Thymidine
- RNA
- Protein Biosynthesis
- Phenylalanine Hydroxylase
- Kinetics
- Insulin
- Enzyme Induction