Phenylalanine hydroxylase activity in foetal hepatic organ culture


Journal Article

The biphasic pattern of phenylalanine hydroxylase (l-phenylalanine tetrahydropteridine:O2 oxidoreductase (4-hydroxylating) EC activity of foetal and neonatal rat liver is not observed in 20- to 22-day-old hepatic explant culture. Two genetic markers of differentiated hepatic tissue were used to monitor hepatic explant: (a) a high baseline tyrosine aminotransferase activity (l-tyrosine:2-oxoglutarate aminotransferase, EC; and (b) hydrocortisone inducibility of this activity. Pre-existing phenylalanine hydroxylase activity of 22-day-old foetal explants decays with a half-life of 6 h. These observations suggest the need for a hepatic or extrahepatic inductive signal for the synthesis of phenylalanine hydroxylase. © 1973.

Full Text

Duke Authors

Cited Authors

  • Tourian, A

Published Date

  • May 5, 1973

Published In

  • Bba Enzymology

Volume / Issue

  • 309 / 1

Start / End Page

  • 44 - 49

PubMed ID

  • 4145353

Pubmed Central ID

  • 4145353

International Standard Serial Number (ISSN)

  • 0005-2744

Digital Object Identifier (DOI)

  • 10.1016/0005-2744(73)90315-X

Citation Source

  • Scopus