The unique identity of rat hepatoma phenylalanine hydroxylase

Published

Journal Article

Rat hepatoma H4-II-E-C3 culture phenylalanine hydroxylase is unique and differs from any of the three isozymes of liver or the one of kidney. Isoelectric focusing results in a single isozyme with an isoelectric point of 5.20 compared to 5.20, 5.30, 5.60 for the liver and 5.35 for the kidney. Hepatoma phenylalanine hydroxylase cross reacts with antibody # 2 of rat liver but differs from isozyme # 2 by a tenfold difference in the ratio of antigen to antibody at equivalence on immunotitration. Kidney phenylalanine hydroxylase which also cross reacts with antibody # 2 of liver can quantitatively (at 2 mU per mg immunoglobulin) absorb antibody against hepatoma enzyme, liver isozyme # 2 and itself without having any effect on antibody # 1 and # 3 of liver. © 1976.

Full Text

Duke Authors

Cited Authors

  • Tourian, A

Published Date

  • January 12, 1976

Published In

Volume / Issue

  • 68 / 1

Start / End Page

  • 51 - 55

PubMed ID

  • 174569

Pubmed Central ID

  • 174569

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(76)90008-5

Citation Source

  • Scopus