Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.

Journal Article (Journal Article)

The hypothesis that there is impaired endogenous synthesis of glucosamine 6-phosphate in Huntington's-chorea fibroblasts was tested by double labelling matched pairs of fibroblasts in culture with carrier-free H3 32PO4 and [U-14C]glucosamine. The [32P]UDP-N-acetyl[14C]glucosamine and [14C]glucosamine 6-[32P]phosphate of the cellular soluble fraction was isolated by charcoal column and paper chromatography. There is no quantitative difference in 32P but a significant difference in 14C in these two sugars in a ratio of approx. 1.5 for Huntington's-chorea fibroblasts compared with normal fibroblasts.

Full Text

Duke Authors

Cited Authors

  • Hung, WY; Tourian, A

Published Date

  • May 15, 1981

Published In

Volume / Issue

  • 196 / 2

Start / End Page

  • 495 - 498

PubMed ID

  • 6459085

Pubmed Central ID

  • PMC1163021

International Standard Serial Number (ISSN)

  • 0264-6021

Digital Object Identifier (DOI)

  • 10.1042/bj1960495


  • eng

Conference Location

  • England