A guide to analysis of mouse energy metabolism.

Journal Article (Journal Article)

We present a consolidated view of the complexity and challenges of designing studies for measurement of energy metabolism in mouse models, including a practical guide to the assessment of energy expenditure, energy intake and body composition and statistical analysis thereof. We hope this guide will facilitate comparisons across studies and minimize spurious interpretations of data. We recommend that division of energy expenditure data by either body weight or lean body weight and that presentation of group effects as histograms should be replaced by plotting individual data and analyzing both group and body-composition effects using analysis of covariance (ANCOVA).

Full Text

Duke Authors

Cited Authors

  • Tschöp, MH; Speakman, JR; Arch, JRS; Auwerx, J; Brüning, JC; Chan, L; Eckel, RH; Farese, RV; Galgani, JE; Hambly, C; Herman, MA; Horvath, TL; Kahn, BB; Kozma, SC; Maratos-Flier, E; Müller, TD; Münzberg, H; Pfluger, PT; Plum, L; Reitman, ML; Rahmouni, K; Shulman, GI; Thomas, G; Kahn, CR; Ravussin, E

Published Date

  • December 28, 2011

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 57 - 63

PubMed ID

  • 22205519

Pubmed Central ID

  • PMC3654855

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

Digital Object Identifier (DOI)

  • 10.1038/nmeth.1806


  • eng

Conference Location

  • United States