Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations.

Journal Article (Journal Article)

Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1.

Full Text

Duke Authors

Cited Authors

  • Bilska, M; Tang, H; Montefiori, DC

Published Date

  • April 2017

Published In

Volume / Issue

  • 33 / 4

Start / End Page

  • 368 - 372

PubMed ID

  • 27758116

Pubmed Central ID

  • PMC5372769

Electronic International Standard Serial Number (EISSN)

  • 1931-8405

Digital Object Identifier (DOI)

  • 10.1089/AID.2016.0142


  • eng

Conference Location

  • United States