Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations.
Journal Article (Journal Article)
Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1.
Full Text
Duke Authors
Cited Authors
- Bilska, M; Tang, H; Montefiori, DC
Published Date
- April 2017
Published In
Volume / Issue
- 33 / 4
Start / End Page
- 368 - 372
PubMed ID
- 27758116
Pubmed Central ID
- PMC5372769
Electronic International Standard Serial Number (EISSN)
- 1931-8405
Digital Object Identifier (DOI)
- 10.1089/AID.2016.0142
Language
- eng
Conference Location
- United States