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Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC.

Publication ,  Journal Article
Tata, PR; Tata, NR; Kühl, M; Sirbu, IO
Published in: Nucleic Acids Res
May 2011

The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the transcription of the mouse EEmiRC locus, presumably through binding of transcription modulators like Oct3/4, Sox2 and CTCF. This IE also contains a CpG island showing a differential pattern of DNA and histone methylation marks during differentiation of ESCs, which places EEmiRC in a novel regulatory feedback loop with DNA methylases. Deletion of IE significantly reduced the transcription of the EEmiRC, further proving the importance of this region in regulating the expression of EEmiRC.

Duke Scholars

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

May 2011

Volume

39

Issue

9

Start / End Page

3574 / 3581

Location

England

Related Subject Headings

  • SOXB1 Transcription Factors
  • Repressor Proteins
  • Pluripotent Stem Cells
  • Octamer Transcription Factors
  • MicroRNAs
  • Mice
  • Epigenesis, Genetic
  • Enhancer Elements, Genetic
  • Embryonic Stem Cells
  • Developmental Biology
 

Citation

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Tata, P. R., Tata, N. R., Kühl, M., & Sirbu, I. O. (2011). Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC. Nucleic Acids Res, 39(9), 3574–3581. https://doi.org/10.1093/nar/gkq1344
Tata, Purushothama R., Nageswara R. Tata, Michael Kühl, and Ioan O. Sirbu. “Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC.Nucleic Acids Res 39, no. 9 (May 2011): 3574–81. https://doi.org/10.1093/nar/gkq1344.
Tata PR, Tata NR, Kühl M, Sirbu IO. Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC. Nucleic Acids Res. 2011 May;39(9):3574–81.
Tata, Purushothama R., et al. “Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC.Nucleic Acids Res, vol. 39, no. 9, May 2011, pp. 3574–81. Pubmed, doi:10.1093/nar/gkq1344.
Tata PR, Tata NR, Kühl M, Sirbu IO. Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC. Nucleic Acids Res. 2011 May;39(9):3574–3581.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

May 2011

Volume

39

Issue

9

Start / End Page

3574 / 3581

Location

England

Related Subject Headings

  • SOXB1 Transcription Factors
  • Repressor Proteins
  • Pluripotent Stem Cells
  • Octamer Transcription Factors
  • MicroRNAs
  • Mice
  • Epigenesis, Genetic
  • Enhancer Elements, Genetic
  • Embryonic Stem Cells
  • Developmental Biology