Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel.

Published

Journal Article

Non-viral gene therapy typically results in low transfection efficiencies and transient gene expression. To address these limitations, two sustained delivery systems capable of releasing functional, compacted DNA for over 50 days were designed. A luciferase plasmid was compacted with a polylysine-polyethylene glycol conjugate and released from agarose hydrogel and lipid microtubule-hydrogel delivery systems for over 50 days. The released DNA was characterized structurally using sedimentation, electron microscopy, and serum stability, and functionally using in vitro transfections. The released DNA retained its physical compaction and nuclease resistance and was converted from supercoiled to nicked and linear forms. Released compacted DNA produced significant gene expression in vitro, although at lower levels than freshly compacted DNA. Thus, hydrogels and lipid microtubules successfully provided the slow release of bioactive, compacted DNA.

Full Text

Duke Authors

Cited Authors

  • Meilander, NJ; Pasumarthy, MK; Kowalczyk, TH; Cooper, MJ; Bellamkonda, RV

Published Date

  • March 2003

Published In

Volume / Issue

  • 88 / 2

Start / End Page

  • 321 - 331

PubMed ID

  • 12628338

Pubmed Central ID

  • 12628338

Electronic International Standard Serial Number (EISSN)

  • 1873-4995

International Standard Serial Number (ISSN)

  • 0168-3659

Digital Object Identifier (DOI)

  • 10.1016/s0168-3659(03)00007-5

Language

  • eng