CS-4,6 is differentially upregulated in glial scar and is a potent inhibitor of neurite extension.

Published

Journal Article

The precise contribution of different CS-GAGs to CSPG-mediated inhibition of axonal growth after CNS injury is unknown. Quantification of the CS-GAGs in uninjured and injured brain (scar tissue) using fluorophore-assisted carbohydrate electrophoresis (FACE) demonstrated that the dominant CS-GAG in the uninjured brain is CS-4 whereas, in glial scar, CS-2, CS-6, and CS-4,6 were over-expressed. To determine if the pattern of sulfation influenced neurite extension, we compared the effects of CS-GAGs with dominant CS-4, CS-6, or CS-4,6 sulfation to intact CSPG (aggrecan), chondroitin (CS-0), and hyaluronan on chick DRG neurite outgrowth. We report that CS-4,6 GAG, one of the upregulated CS-GAGs in astroglial scar, is potently inhibitory and is comparable to intact aggrecan, a CSPG with known inhibitory properties. Thus, a specific CS-GAG that is differentially over-expressed in astroglial scar is a potent inhibitor of neurite extension. These results may influence the design of more specific strategies to enhance CNS regeneration after injury.

Full Text

Duke Authors

Cited Authors

  • Gilbert, RJ; McKeon, RJ; Darr, A; Calabro, A; Hascall, VC; Bellamkonda, RV

Published Date

  • August 2005

Published In

Volume / Issue

  • 29 / 4

Start / End Page

  • 545 - 558

PubMed ID

  • 15936953

Pubmed Central ID

  • 15936953

Electronic International Standard Serial Number (EISSN)

  • 1095-9327

International Standard Serial Number (ISSN)

  • 1044-7431

Digital Object Identifier (DOI)

  • 10.1016/j.mcn.2005.04.006

Language

  • eng