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Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin.

Publication ,  Journal Article
Meilander-Lin, NJ; Cheung, PJ; Wilson, DL; Bellamkonda, RV
Published in: Tissue engineering
March 2005

Prolonging gene expression in skin using safe, nonviral gene delivery techniques could impact skin regeneration and wound healing, decrease infection, and potentially improve the success of tissue-engineered skin. To this end, an injectable, agarose-based delivery system was tested and shown to prolong nonviral gene expression in the skin. DNA was compacted with polylysine to improve DNA stability in the presence of nucleases. Up to 25 microg of compacted luciferase plasmid with or without agarose hydrogel was injected intradermally in rodents. Bioluminescence imaging was used for longitudinal, noninvasive monitoring of gene expression in vivo for 35 days. Injections of DNA in solution produced gene expression for only 5-7 days, whereas the sustained release of compacted DNA from the agarose system prolonged expression, with more than 500 pg (20% of day 1 levels) of luciferase per site for at least 35 days. Southern blotting confirmed that the agarose system extended DNA retention, with significant plasmid present through day 7, as compared with DNA in solution, which had detectable DNA only on day 1. Histology revealed that agarose invoked a wound-healing response through day 14. Tissue-engineering and wound-healing applications may benefit from the agarose gene delivery system.

Duke Scholars

Published In

Tissue engineering

DOI

EISSN

1557-8690

ISSN

1076-3279

Publication Date

March 2005

Volume

11

Issue

3-4

Start / End Page

546 / 555

Related Subject Headings

  • Wound Healing
  • Treatment Outcome
  • Transfection
  • Skin
  • Sepharose
  • Rats, Sprague-Dawley
  • Rats
  • Plasmids
  • Male
  • Hydrogels
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Meilander-Lin, N. J., Cheung, P. J., Wilson, D. L., & Bellamkonda, R. V. (2005). Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin. Tissue Engineering, 11(3–4), 546–555. https://doi.org/10.1089/ten.2005.11.546
Meilander-Lin, Nancy J., Perrin J. Cheung, David L. Wilson, and Ravi V. Bellamkonda. “Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin.Tissue Engineering 11, no. 3–4 (March 2005): 546–55. https://doi.org/10.1089/ten.2005.11.546.
Meilander-Lin NJ, Cheung PJ, Wilson DL, Bellamkonda RV. Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin. Tissue engineering. 2005 Mar;11(3–4):546–55.
Meilander-Lin, Nancy J., et al. “Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin.Tissue Engineering, vol. 11, no. 3–4, Mar. 2005, pp. 546–55. Epmc, doi:10.1089/ten.2005.11.546.
Meilander-Lin NJ, Cheung PJ, Wilson DL, Bellamkonda RV. Sustained in vivo gene delivery from agarose hydrogel prolongs nonviral gene expression in skin. Tissue engineering. 2005 Mar;11(3–4):546–555.

Published In

Tissue engineering

DOI

EISSN

1557-8690

ISSN

1076-3279

Publication Date

March 2005

Volume

11

Issue

3-4

Start / End Page

546 / 555

Related Subject Headings

  • Wound Healing
  • Treatment Outcome
  • Transfection
  • Skin
  • Sepharose
  • Rats, Sprague-Dawley
  • Rats
  • Plasmids
  • Male
  • Hydrogels