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Hydrogel-based three-dimensional matrix for neural cells.

Publication ,  Journal Article
Bellamkonda, R; Ranieri, JP; Bouche, N; Aebischer, P
Published in: Journal of biomedical materials research
May 1995

The ability to organize cells in three dimensions (3D) is an important component of tissue engineering. This study sought to develop an extracellular matrix (ECM) equivalent with a physicochemical structure capable of supporting neurite extension from primary neural cells in 3D. Rat embryonic day 14 striatal cells and chick embryonic day 9 dorsal root ganglia extended neurites in 3D in agarose hydrogels in a gel concentration-dependent manner. Primary neural cells did not extend neurites above a threshold agarose gel concentration of 1.25% wt/vol. Gel characterization by hydraulic permeability studies revealed that the average pore radius of a 1.25% agarose gel was 150 mm. Hydraulic permeability studies for calculating average gel pore radius and gel morphology studies by environmental and scanning electron micrography showed that the average agarose gel por size decreased exponentially as the gel concentration increased. It is hypothesized that the average gel porosity plays an important role in determining the ability of agarose gels to support neurite extension. Lamination of alternating nonpermissive, permissive, and nonpermissive gel layers facilitated the creation of 3D neural tracts in vitro. This ability of agarose hydrogels to organize, support, and direct neurite extension from neural cells may be useful for applications such as 3D neural cell culture and nerve regeneration. Agarose hydrogel substrates also offer the possibility of manipulating cells in 3D, and may be used as 3D templates for tissue engineering efforts in vitro and in vivo.

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Published In

Journal of biomedical materials research

DOI

EISSN

1097-4636

ISSN

0021-9304

Publication Date

May 1995

Volume

29

Issue

5

Start / End Page

663 / 671

Related Subject Headings

  • Rats
  • Polyethylene Glycols
  • Neurons
  • Neurites
  • Neostriatum
  • Microscopy, Electron, Scanning
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Ganglia, Spinal
  • Extracellular Matrix
  • Electrophoresis, Agar Gel
 

Citation

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Bellamkonda, R., Ranieri, J. P., Bouche, N., & Aebischer, P. (1995). Hydrogel-based three-dimensional matrix for neural cells. Journal of Biomedical Materials Research, 29(5), 663–671. https://doi.org/10.1002/jbm.820290514
Bellamkonda, R., J. P. Ranieri, N. Bouche, and P. Aebischer. “Hydrogel-based three-dimensional matrix for neural cells.Journal of Biomedical Materials Research 29, no. 5 (May 1995): 663–71. https://doi.org/10.1002/jbm.820290514.
Bellamkonda R, Ranieri JP, Bouche N, Aebischer P. Hydrogel-based three-dimensional matrix for neural cells. Journal of biomedical materials research. 1995 May;29(5):663–71.
Bellamkonda, R., et al. “Hydrogel-based three-dimensional matrix for neural cells.Journal of Biomedical Materials Research, vol. 29, no. 5, May 1995, pp. 663–71. Epmc, doi:10.1002/jbm.820290514.
Bellamkonda R, Ranieri JP, Bouche N, Aebischer P. Hydrogel-based three-dimensional matrix for neural cells. Journal of biomedical materials research. 1995 May;29(5):663–671.

Published In

Journal of biomedical materials research

DOI

EISSN

1097-4636

ISSN

0021-9304

Publication Date

May 1995

Volume

29

Issue

5

Start / End Page

663 / 671

Related Subject Headings

  • Rats
  • Polyethylene Glycols
  • Neurons
  • Neurites
  • Neostriatum
  • Microscopy, Electron, Scanning
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Ganglia, Spinal
  • Extracellular Matrix
  • Electrophoresis, Agar Gel