Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina.

Published

Journal Article

To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-).Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot.Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression.Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.

Full Text

Duke Authors

Cited Authors

  • Su, X; Tan, QSW; Parikh, BH; Tan, A; Mehta, MN; Sia Wey, Y; Tun, SBB; Li, L-J; Han, X-Y; Wong, TY; Hunziker, W; Luu, CD; Owada, Y; Barathi, VA; Zhang, SS; Chaurasia, SS

Published Date

  • June 2016

Published In

Volume / Issue

  • 57 / 7

Start / End Page

  • 3397 - 3408

PubMed ID

  • 27367508

Pubmed Central ID

  • 27367508

Electronic International Standard Serial Number (EISSN)

  • 1552-5783

International Standard Serial Number (ISSN)

  • 0146-0404

Digital Object Identifier (DOI)

  • 10.1167/iovs.15-18542

Language

  • eng