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Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression.

Publication ,  Conference
Xue, H; Slavov, D; Wischmeyer, PE
Published in: J Biol Chem
November 23, 2012

BACKGROUND: Regulation of transcriptional activity of heat shock factor-1 (HSF1) is widely thought to be the main point of control for heat shock protein (Hsp) expression. RESULTS: Glutamine increases Hsf1 gene transcription in a C/EBPβ-dependent manner and up-regulates HSF1 activity. CONCLUSION: Glutamine is an activator for both HSF1 expression and transactivation. SIGNIFICANCE: Glutamine-induced HSF1 expression provides a novel mechanistic frame for HSF1-Hsp axis regulation. Heat shock transcription factor-1 (HSF1) is the master regulator for cytoprotective heat shock protein (Hsp) expression. It is widely thought that HSF1 expression is non-inducible, and thus the key control point of Hsp expression is regulation of the transactivation activity of HSF1. How HSF1 expression is regulated remains unknown. Herein we demonstrate that glutamine (Gln), a preferred fuel substrate for the gut, enhanced Hsp expression both in rat colonic epithelium in vivo and in cultured non-transformed young adult mouse colonic epithelial cells. This was associated with up-regulation of the transactivation activity of HSF1 via increased HSF1 trimerization, nuclear localization, DNA binding, and relative abundance of activating phosphorylation at Ser-230 of HSF1. More intriguingly, Gln enhanced HSF1 protein and mRNA expression and Hsf1 gene promoter activity. Within the -281/-200 region of the Hsf1 promoter, deletion of the putative CCAAT enhancer-binding protein (C/EBP) binding site abolished the HSF1 response to Gln. C/EBPβ was further shown to bind to this 82-bp sequence both in vitro and in vivo. Gln availability strikingly altered the ratio of C/EBPβ inhibitory and active isoforms, i.e. liver-enriched inhibitory protein and liver-enriched activating protein. Liver-enriched inhibitory protein and liver-enriched activating protein were further shown to be an independent repressor and activator, respectively, for Hsf1 gene transcription, and the relative abundance of these two C/EBPβ isoforms was demonstrated to determine Hsf1 transcription. We show for the first time that Gln not only enhances the transactivation of HSF1 but also induces Hsf1 expression by activating its transcription in a C/EBPβ-dependent manner.

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Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

November 23, 2012

Volume

287

Issue

48

Start / End Page

40400 / 40413

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transcriptional Activation
  • Transcription Factors
  • Rats, Sprague-Dawley
  • Rats
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Mice, Knockout
  • Mice
  • Male
 

Citation

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ICMJE
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Xue, H., Slavov, D., & Wischmeyer, P. E. (2012). Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression. In J Biol Chem (Vol. 287, pp. 40400–40413). United States. https://doi.org/10.1074/jbc.M112.410712
Xue, Hongyu, Dobromir Slavov, and Paul E. Wischmeyer. “Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression.” In J Biol Chem, 287:40400–413, 2012. https://doi.org/10.1074/jbc.M112.410712.
Xue H, Slavov D, Wischmeyer PE. Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression. In: J Biol Chem. 2012. p. 40400–13.
Xue, Hongyu, et al. “Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression.J Biol Chem, vol. 287, no. 48, 2012, pp. 40400–13. Pubmed, doi:10.1074/jbc.M112.410712.
Xue H, Slavov D, Wischmeyer PE. Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression. J Biol Chem. 2012. p. 40400–40413.

Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

November 23, 2012

Volume

287

Issue

48

Start / End Page

40400 / 40413

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transcriptional Activation
  • Transcription Factors
  • Rats, Sprague-Dawley
  • Rats
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Mice, Knockout
  • Mice
  • Male