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Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells.

Publication ,  Journal Article
Khailova, L; Baird, CH; Rush, AA; McNamee, EN; Wischmeyer, PE
Published in: Shock
December 2013

INTRODUCTION: Recent clinical trials show Lactobacillus rhamnosus GG (LGG) administration in critical illness has the potential to reduce nosocomial infections and improve clinical outcome. However, the mechanism(s) of LGG-mediated benefit following illness and injury remain elusive. OBJECTIVE: The aim of this study was to determine the effect of LGG treatment on survival and lung injury in a mouse model of Pseudomonas aeruginosa-induced pneumonia. As increased T regulatory (Treg) cell numbers have been shown to improve outcome in experimental pneumonia, we examined the potential role of Treg cells in probiotic-mediated benefit. METHODS: FVB/N mice were subjected to intratracheal injection of either P. aeruginosa or saline and received LGG or vehicle immediately before procedure. T regulatory cell responses in the lung were evaluated by polymerase chain reaction, Western blotting, and flow cytometry. RESULTS: Mice treated with LGG had significantly improved 7-day survival (P < 0.01) compared with saline-treated control pneumonia mice (55% LGG vs. 14% control). The survival advantage was associated with reduced bacterial counts in bronchoalveolar lavage and with decreased markers of the systemic inflammatory response and improved lung pathology in the probiotic group. Probiotic treatment influenced immune response in the lungs of mice with pneumonia as demonstrated by increased levels of Treg cell marker Foxp3. CONCLUSIONS: These data demonstrate that early administration of LGG improves outcome following P. aeruginosa-induced pneumonia. An effect of LGG on Treg cells may play a role in this protection.

Duke Scholars

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Published In

Shock

DOI

EISSN

1540-0514

Publication Date

December 2013

Volume

40

Issue

6

Start / End Page

496 / 503

Location

United States

Related Subject Headings

  • Treatment Outcome
  • T-Lymphocytes, Regulatory
  • Systemic Inflammatory Response Syndrome
  • Survival Analysis
  • RNA, Messenger
  • Pseudomonas aeruginosa
  • Pseudomonas Infections
  • Probiotics
  • Pneumonia, Bacterial
  • Neutrophil Infiltration
 

Citation

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Khailova, L., Baird, C. H., Rush, A. A., McNamee, E. N., & Wischmeyer, P. E. (2013). Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells. Shock, 40(6), 496–503. https://doi.org/10.1097/SHK.0000000000000066
Khailova, Ludmila, Christine H. Baird, Aubri A. Rush, Eoin N. McNamee, and Paul E. Wischmeyer. “Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells.Shock 40, no. 6 (December 2013): 496–503. https://doi.org/10.1097/SHK.0000000000000066.
Khailova L, Baird CH, Rush AA, McNamee EN, Wischmeyer PE. Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells. Shock. 2013 Dec;40(6):496–503.
Khailova, Ludmila, et al. “Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells.Shock, vol. 40, no. 6, Dec. 2013, pp. 496–503. Pubmed, doi:10.1097/SHK.0000000000000066.
Khailova L, Baird CH, Rush AA, McNamee EN, Wischmeyer PE. Lactobacillus rhamnosus GG improves outcome in experimental pseudomonas aeruginosa pneumonia: potential role of regulatory T cells. Shock. 2013 Dec;40(6):496–503.

Published In

Shock

DOI

EISSN

1540-0514

Publication Date

December 2013

Volume

40

Issue

6

Start / End Page

496 / 503

Location

United States

Related Subject Headings

  • Treatment Outcome
  • T-Lymphocytes, Regulatory
  • Systemic Inflammatory Response Syndrome
  • Survival Analysis
  • RNA, Messenger
  • Pseudomonas aeruginosa
  • Pseudomonas Infections
  • Probiotics
  • Pneumonia, Bacterial
  • Neutrophil Infiltration